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The sorter was triggered on forward scatter at a threshold of 0. Deposited cells were lysed and neutralized followed by whole-genome White pages sanger Uppsala using Phi29 and MDA as described by [ 18 ].

PCR products were detected by an approximate bp fragment on a 1. Altogether, 15 SAGs were selected for genome sequencing. Twelve of these generated swnger 16S rRNA sequence identified by Sanger sequencing and were selected to cover a broad range of phylogenetic groups. Three additional SAGs did not generate any 16S rRNA amplicons with the indicated primers but were nevertheless selected to include also lineages not targeted by bacterial primers.

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The wives of Sweeden version 1. Clustering cutoff for each BACL was set at 0. Out of these, BACL30 resulted in the best alignment at However, none of these two clusters were included in the comparison.

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